Evaluation of CD4~+CD25~+ regulatory T cells and interleukin-4 levels in peripheral blood of asthma

Objective:To investigate the role of CD4+CD25+ regulatory T cells in the pathogenesis of asthma for the purpose of giving new approach of treatment.Methods:The monouclear cells were isolated from peripheral blood of asthmatic patients and control subjects,and the percentagn of CD4+CD25+ regulatory T cells was detemined by flow cytometry.The concentrations of IL-4 in PBMC supernatants were measured with enzyme linked immunosorbent assay (ELISA) method.Results:The percentage of CD4+CD25+ regulatory T cells in PBMC has significantly difference between control group(6.5±2.5)% and asthmatic patients (3.2±1.5)%(P0.05).The concentrations of IL-4 in PBMC supernatants has significantly difference between control group(28.6±7.2) ng/L and asthmatic patients(57.3±13.5) ng/L(P0.05).Conclusion:The CD4+CD25+ regulatory T cells involved in the pathogenesis of asthma in human.