The expression and clinical significance of Caspase-3 and P73 in squamous cell carcinoma of the buccal mucosa before and after chemotherapy

Objective To investigate the expression and clinical significance of Caspase-3 and P73 in squamous cell carcinoma of the buccal mucosa before and after chemotherapy.Methods Thirty cases of squamous cell carcinoma of buccal cancer patients were selected as the experimental group,10 cases of benign tumor of the buccal mucosa as the normal control group.The cancer tissue and normal mucosa were divided into three groups,namely Group A(before chemotherapy),group B(after chemotherapy) and group C(normal mucosa).Use immunohistochemical SP method and flow cytometry to detect Caspase-3 and P73 expression in each group.Results ①Immunohistochemistry: The positive rate of Caspase-3 in the buccal squamous cell carcinoma before chemotherapy was 69.65 ± 2.75%,after chemotherapy was 83.96 ± 3.37%,the positive rate of normal mucosa was 98.53 ± 1.08%;the positive rate of P73 in buccal squamous cell carcinoma before chemotherapy was 89.57 ± 4.0%,after chemotherapy was 71.19 ± 12.3%,the positive rate of normal mucosa was 54.85 ± 4.43%.②Flow cytometry: the expression of Caspase-3 in the buccal squamous cell carcinoma before chemotherapy was 0.715 ± 0.063,after chemotherapy was 0.880 ± 0.038,normal oral mucosal cells was 1.000 ± 0.091.Comparison between the three expression was significant difference(P0.05).The P73 before chemotherapy was 1.310 ± 0.065,after chemotherapy was 1.134 ± 0.037,normal mucosal cells was 1.000 ± 0.091.Comparison between the three expressions was significant difference(P0.05).Conclusion Caspase-3 and P73 may be an early event in buccal mucosa cancer,which can be used as cancer surveillance and a secondary indicator of clinical efficacy.