Application of JEV,PPV,PRRSV and PRV with Multiple PCR

This experiment tested 146 samples of pathogenic tissues from Inner Mongolia, Guangzhou, Guangxi,Tianjin,Beijing and Jilin with JEV and PRRSV double PCR,PPV and PRV double PCR and the four viruses multiple PCR.Among them,7 were PRRSV positive,12 were PPV positive,21 were PRV positive, 4 were PPV and mixed positive. Five JEV samples of artificially infected mice were tested, 4 were positive.Then some of positive products of PCR were used to make dot hybridization and sequence nucleotide.PCR product was confirmed to be correct.The result of Inner Mongolia in China PRRSV positive product sequencing displayed PRRSV was America type.There were three basic radical differences in nucleotide sequence.