Effects of erythropoietin on the expression of dynam in related protein-1 in renal interstitial fibrosis rats

Objective To observe the expression of dynamin related protein-1(Drp-1) of the renal interstitial tissue in rat model induced by unilateral ureteral obstruction(UUO) and the renoprotective effect of erythroprietin.Methods Renal interstitial fibrosis rat model was produced by unilateral ureteral obstruction,eighty-one rats were randomly allocated to shame operation group,UUO model group and treated group.After operation rats in treated group were given subcutaneous injection with erythroprietin 3 000 U·kg-1 once a week until rats were sacrificed.Rats in sham-operated group and UUO model group were given subcutaneous injection with identical normal saline.In each group,nine rats were chosen randomly to be sacrificed at the 7th,14th and 21st day after operation for histological examination.Renal tissues were examined by HE and Masson stain.Immunhistochemistry was performed for Drp-1 in the renal interstitum.Results The renal function(serum Cr and BUN) damage in treatment group was lower than that in the model group at day 21(P0.01),and obviously higher than the sham group(P0.05).Compared with the UUO model group,the score of the renal interstitial damage and fibrotic area of treated group was decreased markedly at three time points(P0.05,respectively).The scores of the renal interstitial damage and fibrotic area of treatment group were higher than those of the sham group(P0.05).The expression of Drp-1 of treated group was decreased markedly at three time points(P 0.05) compared with the UUO model group,and was increased compared with sham group(P0.05).Conclusion The expression of Drp-1 increased during rat renal interstitial fibrosis.Erythroprietin maybe alleviates the degree of renal interstitial fibrosis by down-regulating the expression of Drp-1.