Analysis of Immune Function of Infectious Mononucleosis in Children

Objective To explore the changes in T lymphocyte subsets,B lymphocytes,natural killer(NK)cells and immunoglobulin in different stages of infectious mononucleosis(IM) in children.Methods The flow cytometry and immunoturbidimetry techniques were used to examine blood T lymphocytes CD3,CD4 and CD8,B lymphocytes CD19,NK cells CD56 expressions,and IgG,IgA,and IgM levels of 22 children with IM during the acute phase and recovery phase,and 25 healthy children were selected as healthy control group.Results Children with IM in the acute phase,the peripheral blood CD3,CD4,CD8,CD19,CD56 were(78.71±11.83)%,(18.36±6.06)%,(36.19±8.23)%,(8.15±6.41)% and(18.19±7.61)%,respectively,while in control group they were(60.03±10.22)%,(39.53±8.16)%,(21.55±5.96)%,(19.83±8.49)% and(16.19±6.13)%,respectively.CD3 and CD8 during the acute phase were significantly higher than those in the healthy control group(Pa0.01);CD19 and CD4 levels decreased remarkably,and there were significant differences compared with the healthy control group(Pa0.01);during recovery,the levels of CD3,CD4,CD8,CD19,CD56 were(64.29±10.67)%,(32.14±7.25)%,(25.47±6.07)%,(14.29±7.37)% and(16.75±6.74)%,without significant differences compared to the healthy control group(Pa0.05).Peripheral blood IgG,IgA,IgM in the acute phase of IM children were(17.50±3.92) g·L-1,(1.55±0.36) g·L-1 and(1.27±0.53) g·L-1,while in the healthy control group,they were(7.91±2.82) g·L-1,(0.92±0.28) g·L-1 and(1.09±0.33) g·L-1.In the acute phase of IM,IgG and IgA were obviously higher than those in the healthy control group(P0.01,0.05),IgM showed no obvious difference compared to the healthy control group(P0.05).During recovery stage,the IgG,IgA and IgM levels of IM children were(11.30±3.07) g·L-1,(1.14±0.31) g·L-1 and(1.20±0.35) g·L-1,and IgG level was still higher than that in the healthy control group(P0.05),but IgA and IgM levels had no difference between the both groups(Pa0.05).Conclusions Cellular immunity and humoral immunity disorders have been detected in the children with IM,and the examinations of the immune function of the children with IM can be beneficial for judging the clinical effects,which may provide theoretical basis for the clinical application of immunoregulator.