Detection of HPV infection in cervical Pap smears by TCT test

Objective: The sensitivity and accuracy of the Thinprep pap test (TCT) in diagnosis of human papillomavirus (HPV) infection in cervical Pap smear were evaluated. Methods: Cervical Pap smears obtained from 1030 patients with vulva condyloma acuminata were examined by TCT. The diagnostic criteria were based on The Bethesda System (TBS). Simultaneously HPV DNA in cervical mucus was examined by polymerase chain reaction (PCR) technique and cervical biopsy taken under the guidance of colposcopy for pathological examination. Results: Use PCR to detect the HPV DNA in 1030 samples, 37(35.92%)samples are HPV DNA positive; TCT test 57(5.53%)are ASCUS positive;There are 11 CIN(1%) happened, account for 29.73% of HPV(+) samples, and account for 19.3% of ASCUS positive samples. Conclusion: The results indicates that TCT, a cytologic pathological technique, is a useful method in diagnosis of clinical and subclinical cervical HPV infection.