Effects of Five Macronutrients on the Growth and Flavonoid Accumulation of Cyclocarya paliurus Callus
Xin-Chen Shang-Guan
Abstract
Xin-Chen Shang-Guan
Abstract
The effects of five macronutrients in MS agar medium on the growth and flavonoid accumulation of Cyclocarya paliurus Callus were studied.The results showed that decreasing the concentrations of NH 4NO 3 and KNO 3 to 1/2C in MS medium favored the growth and flavonoids accumulation of callus.In contrast,3/2C KH 2PO 4 was the best concentration of medium,in which the dry weight of callus and flavonoids concentration were 0.52 g/bottle and 4.67%,respectively.The effect of MgSO 4 concentration showed that medium with 3/2C MgSO 4 was suitable for callus growth,while for flavonoid accumulation 1/2C MgSO 4 was better.CaCl 2 had no significant effect on callus growth.However,increasing Ca2+ concentration improved flavonoids accumulation in the medium.
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The effects of five macronutrients in MS agar medium on the growth and flavonoid accumulation of Cyclocarya paliurus Callus were studied.The results showed that decreasing the concentrations of NH 4NO 3 and KNO 3 to 1/2C in MS medium favored the growth and flavonoids accumulation of callus.In contrast,3/2C KH 2PO 4 was the best concentration of medium,in which the dry weight of callus and flavonoids concentration were 0.52 g/bottle and 4.67%,respectively.The effect of MgSO 4 concentration showed that medium with 3/2C MgSO 4 was suitable for callus growth,while for flavonoid accumulation 1/2C MgSO 4 was better.CaCl 2 had no significant effect on callus growth.However,increasing Ca2+ concentration improved flavonoids accumulation in the medium.
Key concepts: Callus, Flavonoid, Chemistry, Botany, Dry weight, Horticulture, Food science, Biology