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2001•Journal of Clinical HematologyRequires access

Effect of cryopreservation on LTC-IC of umbillical cord blood

Bin Zheng

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Abstract

Objective:To explore the effect of cryopreservation on LTC IC counts of human umbilical cord blood.Method:Placental cord blood mononuclear cells were separeted by 6% hetastarch and cryopreserved in liquid nitrogen with 10% DMSO. LTC IC count and CFU GM were detected by stromal contact culture system and CFU GM culture system.Result:After thawing,CFU GM decreased from 97±5/1×10 5 MNC to 84±13/1×10 5 MNC,the recovery was (83±12)%; the number of LTC IC clone also decreased from 87±20/1×10 5MNC to 68±33/1×10 5 MNC,the recovery was (76±33)%.Conclusion:Cryopreservation could bring into some LTC IC lost,but have no influence on their major hematopoietic potentiality.

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What this paper is about

Objective:To explore the effect of cryopreservation on LTC IC counts of human umbilical cord blood.Method:Placental cord blood mononuclear cells were separeted by 6% hetastarch and cryopreserved in liquid nitrogen with 10% DMSO. LTC IC count and CFU GM were detected by stromal contact culture system and CFU GM culture system.Result:After thawing,CFU GM decreased from 97±5/1×10 5 MNC to 84±13/1×10 5 MNC,the recovery was (83±12)%; the number of LTC IC clone also decreased from 87±20/1×10 5MNC to 68±33/1×10 5 MNC,the recovery was (76±33)%.Conclusion:Cryopreservation could bring into some LTC IC lost,but have no influence on their major hematopoietic potentiality.

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Available abstract

Objective:To explore the effect of cryopreservation on LTC IC counts of human umbilical cord blood.Method:Placental cord blood mononuclear cells were separeted by 6% hetastarch and cryopreserved in liquid nitrogen with 10% DMSO. LTC IC count and CFU GM were detected by stromal contact culture system and CFU GM culture system.Result:After thawing,CFU GM decreased from 97±5/1×10 5 MNC to 84±13/1×10 5 MNC,the recovery was (83±12)%; the number of LTC IC clone also decreased from 87±20/1×10 5MNC to 68±33/1×10 5 MNC,the recovery was (76±33)%.Conclusion:Cryopreservation could bring into some LTC IC lost,but have no influence on their major hematopoietic potentiality.

Key concepts: Cryopreservation, Umbilical cord, Cord blood, Peripheral blood mononuclear cell, Andrology, Haematopoiesis, Hetastarch, Chemistry

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