Investigation on the profound hypothermal preservation of human umbilical cord blood stromal cell

Objective To evaluate a simplified method for cryopreservation of human umbilical cord blood stromal cell (HUCBSC). Methods By freezing the cells to -80℃ in low temperature freezer with the combination of 5% DMSO, 3% hydroxyethyl starch (HES) and 4% human serum albumin as cryoprotectant. Observed the cell viability, recovery rate of colony forming unit fibroblasts (CFU F), cell counting and the ability of supporting colony forming(CFU S,CFU GM,CFU E). Result When HUCBSC were preserved at -80℃ as long as 6 months, its cell viability was still (90.6±2.8)%, recovery rate of CFU F reach to (83.6 ±1.8)%.There was no significant difference between pre and after cryopreservation in ability of supporting colony forming.Conclusion This simplified cryopreservation technique is simply and can replace traditional cryopreservation method.