Study on simultaneous determination of benzoic acid and sorbic acid in soy by HPLC
Fengyi Wu
Abstract
Fengyi Wu
Abstract
The analysis method of simultaneous determination of benzoic acid and sorbic acid in soy was discussed by HPLC.Chromatographic conditions were as follows: methanol-0.02 mol/L NH4Ac(5∶95)as the mobile phase,flow rate is 1.0 mL/min,UV wave length of detection is 230 nm,column temperature is 25 ℃ and injection volume is 10 μL.A good linear relationship was obtained by the external standard method in the range of 5~100 μg/mL.The correlation coefficient of regression equation of standard sample were more than 0.999.The detection limit of benzoic acid and sorbic acid were 0.35μg/mL,0.32μg/mL.The average recovery were 94.09% and 98.15%,respectively.The relative standard deviation were 2.09%~3.90%.It shows that this method is accurate,sensitive and simple.
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The analysis method of simultaneous determination of benzoic acid and sorbic acid in soy was discussed by HPLC.Chromatographic conditions were as follows: methanol-0.02 mol/L NH4Ac(5∶95)as the mobile phase,flow rate is 1.0 mL/min,UV wave length of detection is 230 nm,column temperature is 25 ℃ and injection volume is 10 μL.A good linear relationship was obtained by the external standard method in the range of 5~100 μg/mL.The correlation coefficient of regression equation of standard sample were more than 0.999.The detection limit of benzoic acid and sorbic acid were 0.35μg/mL,0.32μg/mL.The average recovery were 94.09% and 98.15%,respectively.The relative standard deviation were 2.09%~3.90%.It shows that this method is accurate,sensitive and simple.
Key concepts: Sorbic acid, Chemistry, Benzoic acid, Chromatography, Detection limit, High-performance liquid chromatography, Correlation coefficient, Relative standard deviation