Study on purification of flavonoids from peanut shells based on Ca~(2+) chelating technology

Using flavonoids of peanut shells and the content of Luteolin as the key parameters,the purification of flavonoids was studied based on the chelating activity of flavonoids with metal ions.The results indicated the optimal purification conditions:the pH of flavonoids extracts solution was 9.0,calcium chloride flavonoids extracts ratio(w/w) was 1:10 and initial concentration of flavonoids extracts was 10.0mg/mL.Under the optimum condition,the content of flavonoids was up to 27.84% from the initial 11.0%.The Luteolin content before and after the purification were determined by HPLC and the analysis revealed that the content of Luteolin increased from 0.87% to 1.97%.