Molecular Marker Techniques and Their Application in the Studies of Soybean Phytophthora Root Rot Disease
Zhang Jun-hua
Abstract
Zhang Jun-hua
Abstract
With the continuously development of genetics,the types and quantities of genetic marker techniques also increased rapidly.Compared with other marker techniques,molecular marker techniques had been applied widely for their superiority.This paper summarized the principle and method of several leading molecular marker techniques such as restriction fragment length polymorphism(RFLP),random amplified polymorphic DNA(RAPD),simple sequence repeats(SSR),amplified fragments length polymorphism(AFLP),sequence characterized amplified region(SCAR),single nucleotide polymorphism(SNP) and sequence related amplified polymorphism(SRAP) and their application in the studies of soybean phytophthora root rot disease.
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With the continuously development of genetics,the types and quantities of genetic marker techniques also increased rapidly.Compared with other marker techniques,molecular marker techniques had been applied widely for their superiority.This paper summarized the principle and method of several leading molecular marker techniques such as restriction fragment length polymorphism(RFLP),random amplified polymorphic DNA(RAPD),simple sequence repeats(SSR),amplified fragments length polymorphism(AFLP),sequence characterized amplified region(SCAR),single nucleotide polymorphism(SNP) and sequence related amplified polymorphism(SRAP) and their application in the studies of soybean phytophthora root rot disease.
Key concepts: RAPD, Amplified fragment length polymorphism, Biology, Restriction fragment length polymorphism, Molecular marker, Genetic marker, Cleaved amplified polymorphic sequence, Root rot