Comparison of Puerarin, Daidzin, Daidzein, Genistein Content of Pueraria lobata,P. thomsonii and P. peduncularis

OBJECTIVE:To compare the content of puerarin, daidzin, daidzein and genistein from Pueraria lobata,P. thomsonii and P. peduncularis. METHODS: After reflux extraction of 30% ethanol, the sample was determined by HPLC. The determination was performed on Uvis-201 (250 mm×4.6 mm,5 μm) column with mobile phase consisted of methanol-water (gradient elution) at the flow rate of 1.0 mL·min-1. The detection wavelength was 250 nm and column temperature was ambient temperature. RESULTS: The linear range was 0.68～5.44 μg for puerarin(r=0.999 2),0.143～1.144 μg for daidzin (r=0.999 3), 0.024 5～0.196 0 μg for daidzein(r=0.999 2) and 0.088～0.704 μg(r=0.999 4) for genistein; average recovery rates were 98.1%,99.5%,101.1% and 99.2%,RSDs were 2.17%,2.42%,2.53% and 2.65%(n=6). The content of puerarin was in descending order:P. lobata in HenanP. peduncularisP. lobata in ShanxiP. lobata in DabieshanP. thomsonii in Guangxi; the content of daidzin was in descending order:P. peduncularisP. lobata in HenanP. lobata in DabieshanP. lobata in ShanxiP. thomsonii in Guangxi; the content of daidzein was in descending order:P. lobata in HenanP. peduncularisP. lobata in ShanxiP. lobata in DabieshanP. thomsonii in Guangxi; the content of genistein was in descending order: P. lobata in HenanP. peduncularisP. lobata in ShanxiP. lobata in DabieshanP. thomsonii in Guangxi. CONCLUSION: The contents of puerarin, daidzin, daidzein and genistein in P. lobata, P. thomsonii and P. peduncularis were different greatly, and P. peduncularis is a false one of P. lobata. The use of them should be different.