Observation of CEA in meningeal carcinomatosis cells of cerebrospinal fluid by laser scanning confocal microscopy

Objective To investigated the cellular distribution and quantitatively of CEA in cerebrospinal fluid meningeal carcinomatosis cells by immunofluorescence staining and laser scanning confocal microscopy (LSCM), explore the diagnostic value of the examination in the patients with meningeal carcinomatosis.Methods All cerebrospinal fluid cells (control group and meningeal carcinomatosis) were stained with CEA by immunofluorescence, scanogram and fluorescent intensity stereophotography were obtained and finally fluorescent values of CEA were individually measured by LSCM.Results Compared with meningeal carcinomatosis cells(44.33±29.58)and control group cells (2.37 ± 2.16), fluorescent value difference CEA was statistically significant (P0.01). Conclusion The sensitivity of CEA by immunofluorescence examination and LSCM is useful for the diagnosis,differential diagnosis of meningeal carcinomatosis.