The application of immunohistochemistry autostainer in anti-hepatic fibrosis

Objective To discuss the application of immunohistochemistry autostainer in anti-hepatic fibrosis.Methods 12 liver fibrosis mice with curcumin treatment collected from January 2012 to April 2012 were divided into observation group and the control group.Immunohistochemistry autostainer was used to stain 6 mice liver tissues of observation groups and manual method was used to stain 6 mice liver tissues of control group.Coloring uniformity,positioning accuracy,the background definition,antibody usage and staining time were used to evaluate the effect of immunohistochemistry autostainer used in immunohistochemistry.Results There were 5 cases of coloring uniformity,6 cases of accurate positioning and 6 cases of clear background,which were significantly more than the control group(P 0.05).Antibody usage was 1.2 ng/cm2 and the average staining time was 15 min,which were significantly lower than the control group(P 0.05).Conclusion Immunohistochemistry autostainer with the advantages of uniform coloring,accurate positioning,clear background,antibodies saving and staining time saving is suitable for pathology researchers and operations staffs to use.