Optimization of Alcohol Precipitation Technology of Changkang Granules Based on Index Components and Pharmacodynamics

Objective: To optimize alcohol precipitation technology of changkang granule, and investigate rationality of alcohol precipitation technology by pharmacodynamics. Method: With impurity removal rate and contents of multi-component( hyperoside, berberine, paeoniflorin and prim-O-glucosylcimifugin) as comprehensive evaluation index,single-factor tests were used to optimize alcohol precipitation technology by taking the concentration of alcohol precipitation and relative density of extract as factors,efficacy of water extract before and after alcohol precipitation was compared through intestinal propulsion test of mice. Result: Optimum alcohol precipitation process was as follows: concentrated extracting liquid to relative density of 1. 13( 70 ℃),added 95% ethanol until the concentration of ethanol to 85%. Conclusion: Good impurity cleaning effect was obtained by optimized alcohol precipitation technology and efficacy was fully kept, it provided experimental basis for scientificity of water extraction-alcohol precipitation technology.