Screening Good Strains by Submerged Culture of Ganoderma lucidum

There are kinds of Ganoderma lucidum.The different cultivars or spices have different productions on their active ingredient.Comparison of mycelium growth rate in submerged culture,fermentation products,fruitbody polysaccharide and mycelium polysaccharide between 8 strains was made.The results showed that:the mycelium growth rates of 8 strains were different,G9 and G.lucidum germinated at first,which grew very quickly.The growth rates between strains were G.sp G.sinenseG8G.mastopdrmaG5760G.atrum.Mycelium balls in submerged culture which were inoculated in PDA medium germinated after about 10 hours,the growth rates between strains were G.lucidumG9G8G.spG.sinenseG.mastopdrmaG5760G.atrum.The growth rate of mycelial balls in submerged culture was higher and better than that of mycelium,thus liquid spawn was used to apply to cultiavate production in order to shorten time and lower cost;Fermentation products of 8 strains were different in biological characterstics,strain biomass was not related to secondary product polysaccharide,the highest biomass was G.sp and the rate of autolysis was the highest,G5760 whose rate of autolysis was the lowest was small mycelium balls.The highest secondary product polysaccharide was G.mastopdrma.;Comparison of fruit body polysaccharide and mycelium polysaccharide between 8 strains,polysaccharide content of mycelium was higher than that of fruit body;In extraction polysaccharide technology,the polysaccharide yield by hot water extraction after ultrasonic treatment was improved significantly,G.sinense and G.atrum improved as twice as that of hot water extraction.