Content Determination of Ursolic Acid and Oleanolic Acid in Kudingcha Species of Aquifoliaceae

[Objective]The research aimed to survey the content of ursolic acid and oleanolic acid in different species and different origins of Kudingcha and provide basic data for the industrialization development of ursolic acid. [Method]The contents of ursolic acid and oleanolic acid in functional leaves of 6 Kudingcha species in Aquifoliaceae and 4 kinds of the marketed shoots products of Ilex kudingcha C. J. Tseng were determined by high performance liquid chromatography (HPLC). The chromatographic conditions were as follows:Eclipse XDB-C18 column(5. 0 μm,4. 6 mm ×150. 0 mm),elution influent methanol -0. 2% aqueous solution of phosphoric acid (88∶12),detection wavelength of 220 nm,flow rate of 0. 8 ml/min,column temperature of 35 ℃ and injection volume of 20 μl. [Result]The contents of ursolic acid in 6 Kudingcha species in Aquifoliaceae were as follows:I. cornuta Lindl. (1. 64% ) I. huoshanesis Y. H. He (1. 63% ) I. latifolia Thunb. (1. 54% ) I. pentagona S. K. Chen,Y. X. Feng et C. F. Liang (1. 23% ) I. kudingcha C. J. Tseng (0. 96% ) I. centrochinesis X. Y. Hu (0. 69% ) the marketed shoots products of Ilex kudingcha C. J. Tseng( 0. 28% ). The contents of oleanolic acid in 6 Kudingcha species in Aquifoliaceae were as follows:I. latifolia Thunb. (0. 22% ) I. huoshanesis Y. H. He (0. 18% ) I. cornuta Lindl. (0. 17% ) I. pentagona S. K. Chen,Y. X. Feng et C. F. Liang (0.14% ) I. kudingcha C. J. Tseng( 0.12%) I. centrochinesis X. Y. Hu (0.10%) the marketed shoots products of Ilex kudingcha C. J. Tseng(0). [Conclusion]The determination results was favorable for guiding the selection of suitable materials for the processing industry of ursolic acid and oleanolic acid.