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2013•Chinese Journal of Animal Infectious DiseasesRequires access

PROKARYOTIC EXPRESSION OF NUCLEOCAPSID PROTEIN OF PORCINE EPIDEMIC DIARRHEA VIRUS

Lunjiang Zhou

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Abstract

In order to illustrate function of nucleoprotein of porcine epidemic diarrhea virus and its application to diagnosis,the N gene fragment was amplified in RT-PCR and cloned into the prokaryotic vector pET-32a for expression in E.coli with induction of IPTG.The expressed protein was examined in SDS-PAGE and determined to be of molecular weight of 60 kDa.The recombinant protein reacted with anti-PEDV hyperimmune serum in Western blot and indirect ELISA.Therefore,the recombinant N protein could be used for further study on PEDV diagnosis and control.

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What this paper is about

In order to illustrate function of nucleoprotein of porcine epidemic diarrhea virus and its application to diagnosis,the N gene fragment was amplified in RT-PCR and cloned into the prokaryotic vector pET-32a for expression in E.coli with induction of IPTG.The expressed protein was examined in SDS-PAGE and determined to be of molecular weight of 60 kDa.The recombinant protein reacted with anti-PEDV hyperimmune serum in Western blot and indirect ELISA.Therefore,the recombinant N protein could be used for further study on PEDV diagnosis and control.

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Available abstract

In order to illustrate function of nucleoprotein of porcine epidemic diarrhea virus and its application to diagnosis,the N gene fragment was amplified in RT-PCR and cloned into the prokaryotic vector pET-32a for expression in E.coli with induction of IPTG.The expressed protein was examined in SDS-PAGE and determined to be of molecular weight of 60 kDa.The recombinant protein reacted with anti-PEDV hyperimmune serum in Western blot and indirect ELISA.Therefore,the recombinant N protein could be used for further study on PEDV diagnosis and control.

Key concepts: Porcine epidemic diarrhea virus, Nucleoprotein, Virology, Recombinant DNA, Biology, Western blot, Virus, lac operon

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