2011•Dongbei Nongye Daxue xuebaoRequires access
Cloning and expression of porcine epidemic diarrhea virus N gene.
Wei Yang, Li GuangXing, Fandan Meng, Xin Wang, Xiaofeng Ren
Open publisher page 0 citations
Abstract
The porcine epidemic diarrhea virus(PEDV) that was isolated from the feces of pigs with diarrhea was named HLJBY and was passaged in Vero cell line.The amplified PEDV N gene was cloned into prokaryotic expression vector PET30a,constructing a recombinant expression vector PET-30a-PEDV-N.High level expression of the N protein was analyzed by SDS-PAGE at different time points at 37 ℃ after IPTG induction.The cultured viruses were purified by ultracentrifugation and injected to a rabbit to generate polyclonal antibody.Western-blotting result showed the polyclonal antibody could specifically bind to PEDV N protein,which indicated that the recombinant fusion protein had excellent immunogenicity.