Karyotypes Analysis of Secale cereale subsp.segetale

The aim of the present study was to provide necessary cytology basis for the evolution,identification,genetic diversity and fine seeds selection of Secale cereale subsp.segetale.The common(slice-making) method combined with micrograph was used to obtain the chromosome number and karyotype of Secale cereale subsp.segetale.Chromosome numbers and karyotype analysis of three populations of Secale cereale subsp.segetale(89R4,89R14,89R60) and one variety Secale cereale L.(H36) were analyzed in this study.Their karyorype formulae were as follows:the karyotype formula of population 89R4 was 2n=2x=14=12m+2sm,where the 7th pair chromosomes was submetacentric,and the rest were metacentric.Its asymmetrical karyotype coefficient was 61.70%.The karyotype formula of population 89R14 was 2n=2x=14=8m+6sm(2sat),where the 5th,6th and 7th pairs chromosomes were submetacentric,and the others were metacentric.The satellites were found at the 7th pair chromosomes in this population.Its asymmetrical karyotype coefficient was 60.89%.The karyotype formula of population 89R60 was 2n=2x=14=14m(2sat).All chromosomes of 89R60 showed metacentric,and the satellites were detected at the 3th pair of the chromosomes.Its asymmetrical karyotype coefficient was 60.47%.The karyotype formula of Secale cereale L.H36 was 2n=2x=14=10m+4sm,where the 5th and 6th pairs chromosomes showed submetacentric,and the others were metacentric,Its asymmetrical karyotype coefficient was 61.20%.