Cloning and Sequence Analysis of c-Myc Gene in Goat

In order to clone sequence of the coding region of the goat c-Myc gene,this study designed specific primers by refering to c-Myc genes coding sequence reported in GenBank including sheep,cattle,pigs,horses,etc.,and then amplified c-Myc coding sequence from embryonic skin tissue of goats by using RT-PCR technique and had sequence analysis.The result indicated that CDS of goat c-Myc gene had 1320bp length including the stop codon.For only 10differences comparing to sheep c-Myc gene nucleotide sequence(GenBank accession number:Z68501.1),homology reached 99.17% and homology of deduced amino acid sequence reached 99.09%.Analysis of homology with other species showed that homology of goat c-Myc gene coding region nucleotide sequence was 91.2%,92.0%,90.2%,86.9%,86.0% with pigs,wolves,humans,rats,mice,and homology of deduced amino acid sequence was 93.6%,96.1%,92.3%,91.1%,89.6%.Using bioinformatics software analysis showed that goat c-Myc protein was localized in the nucleus and contained a HLH domain.This study further researched the function of c-Myc gene and laid the foundation of investigating the effect in the process of goat somatic cell reprogramming.