Cloning,Sequencing and Evolution Analysis of IFN-γ,IL-2 and IL-4 Gene in Chinese White Rabbit

With reverse transcription polymerase chain reaction(RT-PCR),IFN-γ,IL-2 and IL-4 gene were gained from total RNA which was extracted from ConA-stimulated-cultured peripheral blood lymphocytes of Chinese white rabbit.The sequence analysis revealed that:①Compared with one published sequence(sequence number:AB010386),the homology of nucleotide sequence and amino acid sequence of IFN-γgene(sequence number:DQ852341) were both 100%;compared with other mammals in Genbank,including dog,cat,human,pig,cow,horse,goat,giant panda,rat,and so on,the homology of nucleotide sequence was between 63.0%(Rat) and 77.6%(Human),the homology of amino acid sequence was between 41.7%(Rat) and 65.7%(Human);②Compared with one published sequence(sequence number:AF068057),the homology of nucleotide sequence and amino acid sequence of IL-2 gene(sequence number:DQ852342) were 99.6% and 100% respectively;compared with other mammals in Genbank,including dog,cat,human,pig,cow,horse,goat,giant panda,rat,and so on,the homology of nucleotide sequence was between 64.8%(Rat) and 86.0%(Human),the homology of amino acid sequence was between 55.7%(Rat) and 80.1%(Human);③Compared with one published sequence(sequence number:AF169169),the homology of nucleotide sequence and amino acid sequence of IL-4 gene(sequence number:DQ852343) were both 100%;compared with other mammals in Genbank,including dog,cat,human,pig,cow,horse,goat,giant panda,rat,and so on,the homology of nucleotide sequence was between 57.0%(Giant panda) and 69.8%(Human),the homology of amino acid sequence was between 43.0%(Rat) and 53.6%(Human).Phylogenetic analysis based on the nucleotide sequences of the IFN-γ,IL-2 and IL-4 gene respectively showed that rabbit had more closed genetic relation with human,but the farthest with rat.It was consistent with the orthodox taxonomic results,rabbit and rat were belonged to Lagomorpha and Rodentia respectively.