Diagnostic value of combined determination of ANA,anti-dsDNA,anti-ENA polypeptide antibody in autoimmune disease

Objective To explore the diagnostic value of combined determination of ANA,anti-dsDNA,anti-ENA polypeptide antibody in autoimmune disease.Methods Totally 170 patients with AID were enrolled in the investigation.Antinuclear antibody(ANA) and ds-DNA were detected with indirect immunofluorescence assay;anti-ENA polypeptide antibody was detected with immunoblot assay(IBT).The monkey liver tissue and human epithelial cells(Hep-2) were used as antigen substrate for detection of ANA.The test matrix of ds-DNA was Crithidia luciliae.Results The positive rates of ANA were 91.6%,100.0%,100.0%,82.4%,65.4%,35.7%,16.7%,and 42.9% in lupus erythematosus(SLE) group,Sjogren′s syndrome(SS) group,multiple myositis/dermatomyositis(PM/DM) group,mixed connective tissue disease(MCTD) group,progressive systemic scleroderma(PSS) group,rheumatoid arthritis(RA) group,ankylosing spondylitis(AS) group,and allergic purpura(AP) group.The positive rate of anit-ds DNA antibody in SLE group was 65.1%,and the specificity was 96.6%.Anti-ENA polypeptide antibody contributed to the differential diagnosis of AID.Conclusion Detection of ANA with Hep-2/monkey liver tissue as substrate contributes to preliminary screening and identification of AID;detection of anti-ENA antibody repertoire contributes to differential diagnosis of AID;anti-ds DNA antibody may be used a useful marker of curative effect monitoring and prognosis evaluation.