Expression and Purification of Apoptin Gene in E. coil

A novel protein named apoptin induces aapoptosis in various human tumor cells but not in normal cells.To study construction and function of apoptin,recombinant expression plasmid pET-28a-VP3 was constructed.Then the recombinants were transfered into the host strain BL21(DE3) to induce apoptin fusion protein expression by IPTG when OD600 of the culture was about 0.6.The specific protein expressed was detected by SDS-PAGE.The apoptin expression system with pET-28a-VP3 can effectively express apoptin fusion protein.