Osteogenic differentiation of adipose-derived stem cells in mice

Objective Toisolate,cultureandidentifyadipose-derivedstemcells(ADSCs)frommouseanddifferentiate into bone so as to provide a theoretical basis for later experiments which are aim to select suitable seed cells for bone repair. Methods Adipose-derived stem cells were isolated and cultured from white adipose tissue, and surface antigens including CD44 were selected. CD29 and Sca-1 were analyzed by flow cytometry. Differentiation towards adipocyte and osteocyte were determined with different staining methods of alkaline phosphatase, Alizarin red stain and oil-O. Results ADSCs were positive for the pluripotent markers of CD29, CD44 and Sca-1, and able to finish osteogenic and adipogenic differentiation. Conclusions ADSCs can been succeeded to isolate and cultured through the method of collagenase from mouse adipose tissue. The obtained ADSCs could differentiate into diverse cell types. It provides a theoretical basis for later experiments which are aim to repair bone damage tissue by using tissue engineering.