2012•Chinese Journal of Health Laboratory TechnologyRequires access
Establishment and application of Real-Time PCR with probe in detection of salmonella in food poisoning
Zhang Li
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Abstract
Objective:To establish a real-time PCR method for direct amplification of the invA gene of Salmonella in food poisoning.Methods:A representative primer and probe were designed based on invA gene of Salmonella spp..Reaction condition was optimized constantly to test sensitivity and specificity for establishment of Real-Time PCR method.Results: With this PCR method,the sensitivity of salmonella detection in DNA and lysate were 56 fg/PCR system and 9 CFU/ml respectively,the specificity was 100%.15 strains of salmonella were found in the detection of four food poisoning cases,2000 swab anal and 80 food samples.Conclusion: This method had high sensitivity and specificity,which can be used for rapid detection of food poisoning caused by salmonella.