Establishment and Evaluation of Real-time PCR for Food-borne Salmonella Detection

According to the inv A gene(AE008832) of Salmonella from GenBank,a pair of primers were designed to establish a real-time quantitative PCR assay.The correlation coefficient of standard curve was 0.999 and The minimal detection limit was 8 bacteria/reaction.Detecting 40 milk samples and 40 meat samples by real-time quantitative PCR,the positive rate was 2.5% and 5% respectively and the results was identical with the results by conventional microbiology method.Thus,the real-time quantitative PCR is more rapid,sensitive than other methods in detecting Salmonella,and is applicable to investigate and monitor Salmonella contamination in food.