Experimental study on the anti-platelet aggregation and anti-coagulant activity of isoliensinine

OBJECTIVE To investigate the inhibitory effects of isoliensinine on platelet aggregation in rabbits and coagulation function in rats.METHODS Inhibition rates of platelet aggregation for isoliensinine in vivo and vitro were determined by the model of platelet aggregation induced by adenosine diphosphate.The effects of isoliensinine were also evaluated on prothrombin time(PT),activated partial thromboplastin time(APTT) and thrombin time(TT).RESULTS Isoliensinine inhibited platelet aggregation in vitro at the concentration of 4 and 8 mg·L-1,inhibition rates were 14.4% and 27.9%.Isoliensinine inhibited platelet aggregation in vivo at the concentration of 5 and 10 mg·kg-1,inhibition rates were 20.0% and 32.6%.Isoliensinine also markedly prolonged PT,APTT and TT also.CONCLUSION Isoliensinine exerted remarkable effects against platelet aggregation and coagulation in animal study.