Research on Agricultural Bioactivities of Scleroderma flavidum Extracts

[Objective] The bioactivities of S.flavidum extracts on Mythimna separate Walker were studied.[Method] Fruit body of S.flavidum soaked three times by methanol-chloroform(1∶1),merging the extracts and concentrating it under low pressure,the total extract and its petroleum ether,chloroform,butanol extract were obtained;then taking three age larvae of M.separate as experiment materials,the bioactivity were tested.[Result] The results showed that the antifeedant activity of total extract on three age larvae of M.separate was stronger than that of petroleum ether,chloroform,butanol extract.Among the three kinds of extracts,the antifeedant activity of petroleum ether extract was the strongest.The antifeedant rate of petroleum ether extract was 80% at the concentration of 25 mg/ml.While the concentration of petroleum ether extract were over 10 mg/ml,the 24,48 h adjusted mortalities were beyond 70% and 80% respectively,and 7 days adjusted mortalities were all 100%.Contact toxicities of petroleum ether extract on three age larvae of M.separate were weak.[Conclusion] The petroleum ether extract of S.flavidum got stronger stomach poisoning action and antifeedant activities on M.separate;the active ingredients could effectively extracted by petroleum ether.