[hTERT promoter regulated tumor targeting TRALL in cervical cancer cell].

AIM: To investigate the effect of hTERT promoter which regulated tumor targeting TRALL on the cervical cancer cell line HeLa. METHODS: The mRNA expression of TRAIL on HeLa was examined by RT-PCR. The proliferation, apoptosis, motion of the transfected cervical cancer cell were detected by MTT, flow cytometry, and cell invasion assay respectively. The ultrastructure was observed by electron microscope. RESULTS: Compared with the control group, the expression of TRAILmRNA was significantly upregulated in hTERT-TRAIL (P<0.05). The apotosis rate and the inhibitory rate of cell growth of hTERT-TRAIL was significantly high (P<0.05). Electron microscope results indicated that hTERT promoter regulated tumor targeting TRALL facilitated the apoptosis of HeLa. CONCLUSION: The hTERT-TRAIL significantly inhibits the malignant proliferation and invasion ability of the cervical cancer cell line, and facilitates the apoptosisof HeLa, which lays a foundation for the treatment of patients with cervical cancer.