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Impact on growth of Hela cells by chlorpromazine alone or combined with cisplatin

Yaqin Xu

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Abstract

Objective: To investigate the effects of chlorpro-mazinec(CPZ) combined with cisplatinc(DDP) on human cervical carcinoma cell lines Hela.Methods: After treatment of Hela cells with different dose of CPZ、 DDP or combination of both for different time,the inhibitory rate of cells was measured by MTT assay.Cell cycle and apoptosis were analyzed by flow cytometry.The change of cell morphology was observed on different time after medication.Results: CPZ inhibited the cell proliferation with concentration-and time-dependent.CPZ and DDP could induce respectively the apoptosis of Hela cells and cause G0/G1 cell cycle arrest,and this effect of apoptosis induction was increased when combined CPZ with DDP.Conclusion: CPZ can inhibit proliferation of Hela cell,the coordinative effect is observed by combining CPZ with DDP.

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What this paper is about

Objective: To investigate the effects of chlorpro-mazinec(CPZ) combined with cisplatinc(DDP) on human cervical carcinoma cell lines Hela.Methods: After treatment of Hela cells with different dose of CPZ、 DDP or combination of both for different time,the inhibitory rate of cells was measured by MTT assay.Cell cycle and apoptosis were analyzed by flow cytometry.The change of cell morphology was observed on different time after medication.Results: CPZ inhibited the cell proliferation with concentration-and time-dependent.CPZ and DDP could induce respectively the apoptosis of Hela cells and cause G0/G1 cell cycle arrest,and this effect of apoptosis induction was increased when combined CPZ with DDP.Conclusion: CPZ can inhibit proliferation of Hela cell,the coordinative effect is observed by combining CPZ with DDP.

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Available abstract

Objective: To investigate the effects of chlorpro-mazinec(CPZ) combined with cisplatinc(DDP) on human cervical carcinoma cell lines Hela.Methods: After treatment of Hela cells with different dose of CPZ、 DDP or combination of both for different time,the inhibitory rate of cells was measured by MTT assay.Cell cycle and apoptosis were analyzed by flow cytometry.The change of cell morphology was observed on different time after medication.Results: CPZ inhibited the cell proliferation with concentration-and time-dependent.CPZ and DDP could induce respectively the apoptosis of Hela cells and cause G0/G1 cell cycle arrest,and this effect of apoptosis induction was increased when combined CPZ with DDP.Conclusion: CPZ can inhibit proliferation of Hela cell,the coordinative effect is observed by combining CPZ with DDP.

Key concepts: HeLa, Apoptosis, Flow cytometry, Cell cycle, Cisplatin, MTT assay, Cell growth, Chemistry

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