The effect of lipopolysaccharides binding protein inhibitory peptide on CD14 expression of U937 induced by lipopolysaccharides

Objective To investigate the effects of lipopolysaccharides (LPS) binding protein (LBP) inhibitory peptide on CD14 expression on U937 cells induced by LPS.Methods U937 cells were stimulated by Phorbol-myristate-acetate (PMA,100 ng/mL) and divided into five groups:control group,LPS group (100 ng/mL LPS plus 100 ng/mL rhLBP),low dose inhibitory peptide group,middle dose inhibitory peptide group,and high dose inhibitory peptide group (10 ng/mL,100 ng/mL,and 1 000 ng/mL,respectively).The mRNA and protein expressions of CD14 was determined by RT-PCR and western blot and the concentration of TNF-α released from U937 cells was detected by ELISA.Results CD14 mRNA and protein expressions in inhibitory peptide group were lower than those in LPS group and higher than those in control group.The concentration of TNF-α in inhibitory peptide group was lower than that in LPS group and higher than that in control group.Conclusion LBP inhibitory peptide might have a potential protective effect on LPS induced inflammatory disorders such as sepsis and acute lung injury through inhibiting the mRNA and protein expression of CD14 and reducing the release of TNF-α.