2005•Chinese Journal of Critical Care MedicineRequires access
Effects of LBP inhibitory peptide on TLR4 of U937 induced by LPS
XU Debin
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Abstract
Objective To investigate the effects of LBP inhibitory peptide on TLR4 of U937 induced by LPS.Methods U937 was stimulated by phorbol-myritate-acetate( PMA 100 ng/mL) and divided into five groups: control group;LPS group(10 ng/mL LPS+100 ng/mL rhLBP);low dose inhibitory peptide group; middle dose inhibitory peptide group; high dose inhibitory peptide group (10 ng/mL,100 ng/mL and 1 000 ng/mL inhibitory peptide respectively ). The mRNA and protein of CD 14was determined by RT-PCR and Western blot and the concentration of TNFα released from U937 was detected by ELISA.Results Optical density(OD) of TLR4 mRNA and protein in inhibitory peptide group was lower than that in LPS group and higher than that in control group.The concentration of TNFα in inhibitory peptide group was lower than that in LPS group and higher than that in control group.Conclusion LBP inhibitory peptide might have a potential protective role in LPS induced inflammation such as sepsis and acute lung injury by inhibiting the mRNA and protein expression of TLR4 and reducing the release of TNFα.