Influence of Losartan on Proliferation of Cultured Vascular Smooth Muscle Cells with Correlation to Gene Expression of C myc

Objective To explore the effect of losatan (an angiotensin Ⅱ receptor blocking agent) on the vascular remoulding in hypertension and its mechanism. Method ①Cofocusing laser scanning microscope system (Model ACAS570) was used, and by the method of fluorescent immunochemistry, the proliferative cell nuclear antigen(PCNA) and the proliferation related expression product of C myc gene in cultured rat vascular smooth muscle cells treated with losatan were quantitatively assayed. ②The proliferative activity (MTT value) of the vascular smooth muscle cells treated with losartan was analyzed. Result Under the influence of losatan, the vascular smooth muscle cells′ proliferative activity (MTT) decreased, the values in the groups of experiment and control were 0 2 045±0 0 010 and 0 2 239±0 0 010 (P0 05), respectively. The cellular PCNA and the expression of C myc also decreased significantly, PCNA in the groups of experiment and control were 1 338 56±232 56 and 1 542 71±200 04 (P0 01); and C myc expression in the two groups were 1 528 03±125 14 and 1 740 35±205 91(P0 01),respectively. Conclusion Losartan takes part in the changes of the vascular wall structure through effecting on the porliferative activity of vascular smooth muscl cells.