STUDY ON EFFECT OF TOTAL SAPONINS IN SHENMAI ON VASCULAR CELL ADHESION MOLECULE-1 EXPRESSION IN HUMAN UMBILIVAL VEIN ENDOTHELIAL CELL

Objective: To study the effect of total saponins in shenmai on the expression of vascular cell adhesion molecule-1 (VCMA-1) in human umbilical vein endothelial cell(HUVEC) and discuss the possible molecular mechanism of anti-tumor effect of shenmai. Methods: Preparing total saponins from shenmai injection, adopting TNF-α to induce the the expression of vascular cell adhesion molecule-1 (VCMA-1) in human umbilical vein endothelial cell and intervened with a series of concentration of total saponins from shenmai, the gene transcription and protein expression of vascular cell adhesion molecule-1 was determined by fluorescence quantitative PCR and flow cytometry. Results: TNF-α could induce HUVEC to express VCMA-1 in the shape of dose-effect relationship, 20ng/ml was the adequate induce concentration; total saponins in shenmai could inhibit the gene transcription and protein expression of vascular cell adhesion molecule-1 in HUVEC induced by TNF-α, the effect present positive correlation, but there was no effect on VCMA-1 expression in normal HUVEC. Conclusion: Total saponins in shenmai can inhibit the expression of vascular cell adhesion molecule-1 in human umbilical vein endothelial cell induced by TNF-α, which suggests that total saponins in shenmai can control the cancerometastasis.