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Research on Induction and Regeneration of Callus Initiated from Seedling-derived Young Leaf Segments of Maize

Dong Shuting

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Abstract

The initiation,maintenance and differentiation of callus from seedling-derived leaf segments of six maize genotypes had been studied.It was revealed that the optimum concentration of 2,4-D for the leaf segments-induced callus was 3 mg/L.Callus induction and regeneration were dependent on the genotypes.Due to their better performance in callus induction and morphology,embryogenic callus of Qi319,Mo17 and Luyuan 92 were transformed with a GUS construct.The transient expression of GUS was performed by the histochemical staining assay.The results showed that the GUS+ frequency of young leaf segments-derived callus was high.This study provides technological reference for selection of leaf segment as an efficient explant for maize regeneration and genetic transformation.

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What this paper is about

The initiation,maintenance and differentiation of callus from seedling-derived leaf segments of six maize genotypes had been studied.It was revealed that the optimum concentration of 2,4-D for the leaf segments-induced callus was 3 mg/L.Callus induction and regeneration were dependent on the genotypes.Due to their better performance in callus induction and morphology,embryogenic callus of Qi319,Mo17 and Luyuan 92 were transformed with a GUS construct.The transient expression of GUS was performed by the histochemical staining assay.The results showed that the GUS+ frequency of young leaf segments-derived callus was high.This study provides technological reference for selection of leaf segment as an efficient explant for maize regeneration and genetic transformation.

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Available abstract

The initiation,maintenance and differentiation of callus from seedling-derived leaf segments of six maize genotypes had been studied.It was revealed that the optimum concentration of 2,4-D for the leaf segments-induced callus was 3 mg/L.Callus induction and regeneration were dependent on the genotypes.Due to their better performance in callus induction and morphology,embryogenic callus of Qi319,Mo17 and Luyuan 92 were transformed with a GUS construct.The transient expression of GUS was performed by the histochemical staining assay.The results showed that the GUS+ frequency of young leaf segments-derived callus was high.This study provides technological reference for selection of leaf segment as an efficient explant for maize regeneration and genetic transformation.

Key concepts: Callus, Seedling, Explant culture, Biology, Regeneration (biology), Transformation (genetics), Botany, Horticulture

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Research on Induction and Regeneration of Callus Initiated from Seedling-derived Young Leaf Segments of Maize — Research Paper | ScholarLens