Determination of polysaccharides content of Gynostemma pentaphyllum(Thunb.) Makino from different producing areas

Objective To establish a method for the determination of polysaccharides in Gynostemma pentaphyllum(Thunb.)Makino,and prepare the difference of content from different producing areas.Methods Polysaccharides was extracted and purified from Gynostemma pentaphyllum(Thunb.)Makino by water extraction and alcohol precipitation,and the content of polysaccharides was determined by anthrone-sulphuric acid colorimetry.Results Polysaccharides content of Gynostemma pentaphyllum(Thunb.)Makino from different producing areas was 3.26%,6.38%,3.99%,4.28%,4.86%,2.65%,respectively.Conclusion The method for determinating polysaccharides was simple,stable and workable,polysaccharides content was significantly different from different producing areas,the results provided references for polysaccharides content quality control and developing and using it.