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2002•Chieh P'ou Hsueh PaoRequires access

A METHOD OF THE ARTIFICIAL LIPOFUSCIN PREPARATION

Xiao Changyi

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Abstract

Objective To investigate the method of the artificial lipofuscin preparation. Methods Extracting the hepatic mitochondrion of rat, we applied oxidation-reduction method to making and obtaining the artificial lipofuscin.The capability of artificial lipofuscin being digested by peritoneal macrophages of mice was examined. Results The artificial lipofuscin had positive reactions both to specific histochemical stain and FITC fluorescence label.Positive granules or fluorescence were present in cytoplasm of the macrophages and its distribution was similar to that of positive control.Conclusion The artificial lipofuscin had the same histochemical character and biological effect as natural lipofuscin, so it could be used as a substitute for anti-senescence research.

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What this paper is about

Objective To investigate the method of the artificial lipofuscin preparation. Methods Extracting the hepatic mitochondrion of rat, we applied oxidation-reduction method to making and obtaining the artificial lipofuscin.The capability of artificial lipofuscin being digested by peritoneal macrophages of mice was examined. Results The artificial lipofuscin had positive reactions both to specific histochemical stain and FITC fluorescence label.Positive granules or fluorescence were present in cytoplasm of the macrophages and its distribution was similar to that of positive control.Conclusion The artificial lipofuscin had the same histochemical character and biological effect as natural lipofuscin, so it could be used as a substitute for anti-senescence research.

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Available abstract

Objective To investigate the method of the artificial lipofuscin preparation. Methods Extracting the hepatic mitochondrion of rat, we applied oxidation-reduction method to making and obtaining the artificial lipofuscin.The capability of artificial lipofuscin being digested by peritoneal macrophages of mice was examined. Results The artificial lipofuscin had positive reactions both to specific histochemical stain and FITC fluorescence label.Positive granules or fluorescence were present in cytoplasm of the macrophages and its distribution was similar to that of positive control.Conclusion The artificial lipofuscin had the same histochemical character and biological effect as natural lipofuscin, so it could be used as a substitute for anti-senescence research.

Key concepts: Lipofuscin, Stain, Chemistry, Biochemistry, Cytoplasm, Staining, Biology, Genetics

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