2013•Medical Entomology and ZoologyOpen access
Autologous fluorescence exclusion method of lipofuscin in a brain tissue section
Masako Sogo, Yukiko Kuroda, Miki Fujimoto, Takao Mitsui
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Abstract
Lipofuscin has autologous fluorescence. When an immunofluorescent stain is applied to an autopsy brain tissue specimen, it is necessary to erase the autologous fluorescence of the lipofuscin. However, in the conventional report, it was difficult to remove lipofuscin enough. We examined the autologous fluorescence removal of lipofuscin in brain tissue using various methods. The autologous fluorescence could be completely removed by using Autofluorescence Eliminator Reagent R for 45 minutes.