2010•Harbin Yike Daxue xuebaoRequires access
Package of recombinant adenovirus of SHP-2 and transfection to the cardiomyocytes
Hong Li
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Abstract
Objective To package Ad-GFP-SHP-2 and Ad-GFP-SHP-2-E76A and amplify Ad-GFP and transfect viruses to neonatal rat cardiomyocytes.Methods Two vectors were purified and digested by Pac Ⅰenzyme.The linearized vector was transfected into HEK293 cells.Adenovirus particles were isolated from HEK293 cells and transfected into neonatal rat myocytes.Results A 4.5 kb band was shown on agarose gel after PacⅠdigestion.The supernatant of HEK293 cells contained adenovirus particles.When MOI was 100,transfection efficiency was more than 90%.Conclusion Recombinant SHP-2 adenoviruses were packaged and neonatal rat cardiomyocytes can be efficiently tranfected by the viruses.