Transfection efficiencies of adenoviruses Ad5-GFP,Ad5F35-GFP and Lipofectamine~(TM) 2000 to four cell lines

Objective To comparing the transfection efficiencies adenoviruses Ad5-GFP,Ad5F35-GFP and LipofectamineTM 2000 to four cell lines so as to obtain the suitable cell line for Ad5F35-GFP and the suitable transfection method for various cells.Methods Human chronic granulocytic leukemia K562 cells,human peripherhal blood mononuclear cells(PBMCs),mouse adipocyte 3T3-L1 strain and mouse liver cancer Hep A 1-6 cells were transfected with adenoviruses Ad5-GFP,Ad5F35-GFP and plasmid pEGFP-N1(with LipofectamineTM 2000),separately.The transfection efficacy was observed under invert fluorescent microscope 48 h after transfection,while the transcription level of GFP mRNA was determined by RT-PCR.Results Ad5F35-GFP was transfected to K562 cells and PBMCs effectively,with transfection efficacies of 61% and 56% respectively,while was not effectively transfected to 3T3-L1(5%) and Hep A1-6(15%) cells.However,Ad5F35-GFP,Ad5-GFP and LipofectamineTM 2000 were not effectively transfected to 3T3-L1 cells,with transfection efficacies of 5%,5% and 6% respectively.The transfection efficacies of Ad5-GFP and Lipofectamine%TM 2000 to Hep A1-6 cells were 73% and 71% respectively.Conclusion Ad5F35-GFP may be effectively transfected to human hemopoietic cells and monocytes,which laid a foundation of study on relevant genes and their application in gene therapy.