The analysis of the bile component following interposition of gallbladder between the common hepatic duct and duodenum

Objective To investigate the change of the bile and its clinical implication following the gallbladder interposition between the common hepatic duct and duodenum for bile duct reconstruction in animal models. Methods This experiment was carried out on 15 dogs. Of them, 5 were subjected to the single gallbladder puncture and 10 received the gallbladder interposition between the common hepatic duct and duodenum, and then underwent the gallbladder puncture 12 weeks after operation. The bile were analyzed including bile routine examination, bile culture, and quantitative determination of the bile components [total bile acid (TBA), cholesterol, total bilirubin (TB) and inorganic salts (Na +, K +) during operation. Results All animals were alive at termination of the study. Bile routine examination was negative in the two groups. Esherichia coli was cultured in half of the bile in the experimental group and nothing in the control group. The concentration of TBA in the experimental group was significantly increased as compared with that of control group ( P 0.01), while the concentrations of cholesterol and TB decreased ( P 0.05). The concentrations of K + and Na + were significantly decreased ( P 0.01). Conclusion The concentration of TBA was significantly increased after the gallbladder interposition for bile duct reconstruction, suggesting the gallbladder preserved the function of concentration. The concentrations of cholesterol, TB and inorganic salts were decreased, indicating the lower morbidity of cholelithiasis.