PTEN and CDK6 Proteins Expression in Endometrioil Carcinoma

Objective To study the expression of PTEN and CDK6 proteins in endometrioil carcinoma and its clinical significance.Methods The expression of PTEN and CDK6 proteins were tested by S-P immunohistochemistry on tissues of normal endometrium(30 cases),endometrial hyperplasia(30 cases),atypical hyperplasia(30 cases)and endometrioil carcinoma(45 cases).Results For atypical hyperplasis and endometrioid carcinoma groups,the positive expression rate of PTEN and CDK6 proteins was 56.7%,46.7%,40.0% and 66.7%,respectively.The positive expression of PTEN was significantly lower(Ρ0.001)and CDK6 was significantly higher(Ρ0.003)than those of normal endometrium and endometrial hyperplasia groups.The expressions of PTEN and CDK6 proteins were negatively correlated in atypical hyperplasia and in endometrioid carcinoma groups(r=-0.384 5,P0.04;r=-0.472 5,P0.002).In endometrioid carcinoma group the loss of positive expression of PTEN protein was significantly associated with histological grade(Ρ0.04),muscles invasion(Ρ0.02)and clinical stage(Ρ0.03).The positive expression of CDK6 protein was significantly correlated with muscles invasion(Ρ0.05)and clinical stage(P0.03).The lower expression of PTEN and the higher expression of CDK6 were significantly related with recurrence of endometrioid carcinoma(Ρ0.05,Ρ0.02).Conclusion The loss expression of PTEN and the over-expression of CDK6 protein are closely related to the generation and development of medometrioid carcinoma.Combined detections of PTEN and CDK6 proteins might be a useful biomarker of clinical prediction of tumor recurrence.