Active components in six kinds of ground bamboo leaves and their anti-oxidant activities

Objective To study the effective components of the leaves in six kinds of ground bamboo(Shibataea chinensis,Pleioblastus kongosanensis f.aureostriatus,Indocalamus latifolius,Sasa pygmaea,S.argenteastriatu,and S.veitchii) and their anti-oxidant activities.Methods The contents of total phenolics,total flavonoids,and triterpenoids in bamboo leaves and their anti-oxidant activities were detected and investigated by using Forint reduction assay reagent,aluminum nitrate-sodium nitrite colorimetry,and vanillin-acetic acid assay,respectively.Eight characteristic compounds(orientin,isoorientin,vitexin,homovitexin,p-coumaric acid,chlorogenic acid,caffeic acid,and ferulic acid) were quantitatively analyzed by UPLC synchronously.The anti-oxidative and reducing activities of extracts from bamboo leaves were investigated by DPPH and FRAP.Results Six kinds of ground bamboo leaves contained abundant botanic secondary metabolites.The content of total phenolics,on dry basis of leaves,varied in a range of 4.15%―9.12% in the six kinds of ground bamboo,total flavonoids and triterpenoids located in 1.62%―4.00% and 0.44%―0.57%,respectively.The sequence of the eight characteristic compounds in total phenolics was S.veitchii 6.81% S.argenteastriatus 4.80% S.pygmaea 4.35%,P.kongosanensis 4.35% S.chinensis 1.54% I.latifolius 1.08%.The content of eight characteristic compounds and their anti-oxidant activities in each ground bamboo leaves were significantly changed(P 0.05).Conclusion Among the six kinds of ground bamboo,there are much more effective components and more outstanding anti-oxidant activities of radical scavenging and iron reduction in two species(S.chinensis and P.kongosanensis) than those in any other banboos.