The effect of Sijunzi decoction before and after fermentation on cell proliferation of splenic lymphocyte of mice in vitro

Objective To investigate the immunomodulatory activity of decoction of Sijunzi fermented with microorganisms.Methods Effect of Sijunzi decoction before and after fermentation on lymphocyte proliferation in vitro was investigated with MTT assays to evaluate the impact of fermentation on the function of the decoction.Results The optimal concentration was 1,600 μg/mL for Sijunzi decoction and 800 μg/mL for fermented decoction of Sijunzi to most obviously enhance the lymphocyte proliferation.Conclusion The fermented decoction of Sijunzi is more effective in improving lymphocyte proliferation than unfermented Sijunzi decoction.