Experimental study on anti-tumor effect of sijunzi decoction

Objective:To study the anti-tumor effect of sijunzi decoction on expermental tumor mice,and to clarify the mechanisms of the anti-tumor effect of sijunzi decoction.Methods:In vivo experiment,established mice bearing neoplasm(SRS-82 cell line)as the experimental tumor model,the mice were fed with sijunzi decoction.After 15 treatment days,the mice were killed.The neoplasm block was stripped and weighed,and the inhibition rate of the anti-tumor was calculated. We use electronic microscope to observe the neoplasm cells, and extract the DNA of the cells for agarose gel electrophoresis.Results:The weights of the neoplasm of sijunzi decoction group are significant lighter than those the control groups (P0.01). But we do not find that the tumor cells of sijunzi decoction group appear special alteration which infer apoptosis. Conclusion: Sijunzi decoction has obvious anti-tumor effect in experimental tumor model.Sijunzi decoction has no effect to induce the tumor cells apoptosis.