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2008•Chinese HepatologyRequires access

Relationship between hepatitis B virus basic core promoter mutations and viral genotypes

WU Yue-re

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Abstract

Objective To investigate the relationship between hepatitis B virus(HBV) basic core promoter (BCP) mutations and viral genotypes.Methods Sera from 67 patients chronically infected with HBV were tested. HBV genotype was determined by real-time polymerase chain reaction (PCR) using TaqMan-MGB probe technology. Double-mutation in BCP(T1762/A1764) were determined by PCR assay and direct DNA sequencing. Results The double-mutation in BCP(T1762PA1764) was significantly more frequent in genotype C patients than that in genotype B patients(52.2 % vs 25.0 % ,χ2=4.179 , P0.05).Conclusion Genotype C and genotype B are the two dominant genotypes in patients living in Suzhou area. Compared with genotype B , the double-mutation in BCP(T1762/A1764) was more common in patients with genotype C patients.

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Objective To investigate the relationship between hepatitis B virus(HBV) basic core promoter (BCP) mutations and viral genotypes.Methods Sera from 67 patients chronically infected with HBV were tested. HBV genotype was determined by real-time polymerase chain reaction (PCR) using TaqMan-MGB probe technology. Double-mutation in BCP(T1762/A1764) were determined by PCR assay and direct DNA sequencing. Results The double-mutation in BCP(T1762PA1764) was significantly more frequent in genotype C patients than that in genotype B patients(52.2 % vs 25.0 % ,χ2=4.179 , P0.05).Conclusion Genotype C and genotype B are the two dominant genotypes in patients living in Suzhou area. Compared with genotype B , the double-mutation in BCP(T1762/A1764) was more common in patients with genotype C patients.

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Available abstract

Objective To investigate the relationship between hepatitis B virus(HBV) basic core promoter (BCP) mutations and viral genotypes.Methods Sera from 67 patients chronically infected with HBV were tested. HBV genotype was determined by real-time polymerase chain reaction (PCR) using TaqMan-MGB probe technology. Double-mutation in BCP(T1762/A1764) were determined by PCR assay and direct DNA sequencing. Results The double-mutation in BCP(T1762PA1764) was significantly more frequent in genotype C patients than that in genotype B patients(52.2 % vs 25.0 % ,χ2=4.179 , P0.05).Conclusion Genotype C and genotype B are the two dominant genotypes in patients living in Suzhou area. Compared with genotype B , the double-mutation in BCP(T1762/A1764) was more common in patients with genotype C patients.

Key concepts: Genotype, Virology, Hepatitis B virus, TaqMan, Polymerase chain reaction, Mutation, Biology, Virus

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