Determination of Content and Entrapment Efficiency of Curcumin Albumin Nanoparticles by HPLC

Objective To establish a method for determining the content and entrapment efficiency of curcumin albumin nanoparticles.Methods The separation was performed on a Hypersil C18 column(250 mm×4.6 mm,5 μm) with a mobile phase of 4% glacial acetic acid-acetonitrile(45:55).The process was carried out with a flow rate of 1.0 mL·min-1,column temperature at 30 ℃ and wavelength at 425 nm.Refrigerated high-speed centrifugation method was employed to isolate free curcumin for the entrapment efficiency determination.Results The calibration curve was linear in the range of 0.102-16.32 μg·mL-1(r=0.999 9).The mean recovery(n=9) was(99.96±1.01) %.The mean content of labeled amount was(100.31±0.94) %,and encapsulation efficiency was(71.13±1.30) %.Conclusion The method is sensitive,simple,accurate and applicable for determining entrapment efficiency of curcumin in the albumin nanoparticles.