Pharmacokinetics Comparison between Puerarin Nanoparticles Water Dispersion System and Puerarin Suspension in Mice

Objective:To determine the plasma concentration of mice administered with puerarin nanoparticles water dispersion system and puerarin suspension,to calculate the pharmacokinetic parameters and compare the pharmacokinetic process of different diameter particles.Methods: The mice were administered with puerarin nanoparticles water dispersion system and puerarin suspension and the plasma concentration of different time were determined by high performance liquid chromatography(HPLC).The pharmacokinetic parameters were fitted by 3P87 software.Results: The plasma concentration-time curve was fitted to a two-compartment pharmacokinetic model.The main pharmacokinetic parameters of puerarin nanoparticles water dispersion system and puerarin suspension were as follows: t1/2 Ke was 2.54 h and 0.52 h,AUC was 8.20 μg·h/ml and 2.24 μg·h/ml,CLS was 609.00 ml·kg-1/min and 1 488.00 ml·kg-1/min,Cmax was 1.8 μg/ml and 1.62 μg/ml,respectively.The difference of main pharmacokinetic parameters was significant.Conclusion: Puerarin nanoparticle water dispersion system is eliminated more slowly than puerarin suspension,nanoparticles system has a certain prolonged action.